Oral Presentation CD1-MR1 Workshop 2025

iNKT cells: versatile platforms for direct and recombinant antigen receptor-mediated cancer therapies alone and in rational immunotherapy combinations (#68)

Mark Exley 1 , Marco Purbhoo 1 , Terese C Hammond 2 , Samuel L Cytryn 3 , Yelena Y Janjigian 3 , Manuel Hidalgo 4 , Martyna Popis 1 , Efrat Altman-Sharoni 1 , Magdalena Niedzielska 1 , Paul Ibbett 1 , Amy Chlamers 1 , Stephen Addis 1 , Olivier le Tonquesse 1 , Eleni Chantzoura 1 , Mark van Dijk 1 , Jennifer S Buell 1 , Nils-Petter Rudqvist 1
  1. MiNK Therapeutics Inc, PHILADELPHIA, PA, United States
  2. Medicine, Kaweah Health Medical Center, Los Angeles, CA, USA
  3. Oncology, Memorial Sloan Kettering, New York, NY, USA
  4. Weill-Cornell Medical Center, New York, NY, USA

Despite significant advances in cancer therapies, many malignancies remain resistant to current treatments due to immunosuppressive mechanisms, limited neoantigen expression and dynamic tumor adaptations. Furthermore, multiple life-threatening infectious &/or inflammatory conditions threaten many patients, underscoring the need for innovative therapeutic widely-usable strategies. iNKT cells have inherent anti-tumor, anti-pathogen and immunomodulatory properties.  Unlike classical T cells, human iNKT can proliferate logarithmically without exhaustion.  They can also be transduced with CARs and rTCRs to enhance targeting. 

We have produced a hghly-pure allogeneic iNKT cell therapy called agent-797.  We have an in-house, scalable, closed automated process to produce functional iNKT at >100 billions of cells/donor and relatively low cost-of-goods.  agent-797 has been used to effectively and safely treat patients with acute respiratory distress trial with various causes in an ongoing clinical (n > 40).  agent-797 has also been used in promising clinical trials in cancer patients, alone and in combination with checkpoint antibodies (n > 45).  Combination of agenT-797 with other therapies induced tumor infiltration by T cells and antigen-presenting cells, activation of central and effector memory T cells and elevated levels of circulating IFN-γ. Additional biomarker analyses are in process, including TCR sequencing of tissue and matched peripheral blood.  Latest data will be presented.  In addition, cancer stromal FAP-targeted CAR was expressed in IL15-armored iNKT (MiNK-215) and a PRAME-HLA-A02-targeted recombinant TCR also expressed in iNKT.  Both specifically enhanced relevant anti-tumor activities of iNKT cells in vitro and in vivo and are being developed for further trials.  Functional data will also be shown.  

  1. Hammond TC, Purbhoo MA, Kadel S, Ritz J, Nikiforow S, Daley H, et al. A Phase 1/2 Clinical Trial of Invariant Natural Killer T Cell Therapy in Moderate-Severe Acute Respiratory Distress Syndrome. Nat Commun (2024) 15(1):974. Epub 2024/02/07. doi: 10.1038/s41467-024-44905-z. Cytryn et al. in prep.