In recent years, there has been increasing interest in CD4-/CD8- double-negative αβ T cells (DN T cells) due to their clinical significance in autoimmune conditions, as well as their proposed potential in anti-tumor immunity and transplant tolerance. These seemingly contradictory proinflammatory and immunoregulatory functions of DN T cells are likely due to the fact that they are not a homogenous T cell subset, but instead consist of multiple T cell populations that differ in specificity and function. Indeed, we determined by single cell RNA-seq and TCR-seq that DN αβ T cells in healthy donors harbor multiple populations with distinct gene expression profiles. As expected, both iNKT cell and MAIT cell populations were represented among DN T cells, and both populations expressed KLRB1 (encoding CD161, a C-type lectin) and ZBTB16 (encoding PLZF, a key transcription factor directing the development and function of innate-like T cells). Distinct from iNKT cells and MAIT cells, we identified a novel population of DN T cells that also expressed KLRB1 and ZBTB16, indicating the presence of a previously uncharacterized population of innate-like DN T cells. In contrast to iNKT cells and MAIT cells, this population did not harbor invariant TCR(s), but did preferentially express the TRBV4-1 variable region, which has previously been associated with CD1b and CD1c-restricted T cells, suggesting that CD1b or CD1c may be a potential restriction element for these T cells. Ongoing efforts are focused on linking this newly identified innate-like T cell population to TCR restriction, and understanding their function based on a unique gene expression profile.