Mucosal-associated invariant T (MAIT) cells protect against lung injury, but their spatial interactions and mechanisms of action remain poorly defined. Using single-cell resolution spatial transcriptomics (MERSCOPE), we profiled over 1.8 million cells from 14 lung tissue sections collected across seven time points from bleomycin-treated wild-type and MAIT cell-deficient Mr1⁻/⁻ mice. We identified seven conserved cellular niches, with alveolar niches reducing, immune and fibrotic niches expanding after injury. Fibrotic niches featured fibroblast–macrophage co-localisation, with interstitial macrophages (IM) expressing both pro-fibrotic and reparative signatures. B cell follicles emerged in immune niches, showing CXCL13 expression in B cells, goblet cells and CD4⁺ T cells. cDC1, regulatory T cells, and γδ T cells were spatially enriched near MAIT cells. cDC1s close to MAIT cells upregulated activation markers such as Xcr1, Flt3, and Ccr2, while nearby T cells expressed higher levels of supression marker Ctla4. Spatial analysis (using Spacia) revealed that MAIT cell-derived Ctla4, Pdcd1, and Tgfb1 were associated with reduced expression of pro-inflammatory genes (Il6, Ccl2, Ereg) in surrounding immune cells, particularly cDC1 and naïve CD4⁺ T cells. Re-analysis of human pulmonary fibrosis spatial transcriptomic data confirmed the presence of CXCL13⁺ B cell follicles and an increased MAIT cell gene signature in fibrotic lungs, mirroring murine findings. Together, this work provides the first spatially resolved map of MAIT cell interactions in lung injury and highlight their role in modulating local immune responses.