The eye is the only organ responsible for visual function, yet the mechanism by which maintains its integrity is not well understood. Herein, studying an experimentally autoimmune uveitis (EAU) mouse model that accompanies reduced visual function, we observed that mucosal-associated invariant T (MAIT) cells have protective roles of visual function. Single cell analysis of EAU-induced retina and intravitreal administration experiments with MAIT cell agonist 5-OP-RU [5-(2-oxopropylideneamino)-6-D-ribitylaminouracil] and antagonist Acetyl-6-formylpterin (Ac-6-FP) to EAU mice showed involvement of TCR-dependent MAIT cell activation on this protective effect. Spatial transcriptomic analysis revealed that Wingless/int1(Wnt) signaling pathway-related genes were upregulated in the retina of EAU-induced mice in a MR1-dependent manner. Intravitreal administration of a Wnt signaling agonist improved visual function in EAU mice. These results reveal a protective immunological response mounted by MAIT cells in the eye.